CHARACTERIZATION OF PORCINE PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS GAMMA-1 AND GAMMA-2 - DETECTION OF BREED AND AGE-DIFFERENCES IN GENE-EXPRESSION

Two isoforms of peroxisome proliferator-activated receptor gamma (PPAR gamma) cDNAs, gamma 1 and gamma 2, have been isolated and characteris ed in swine. The relative expression of the two transcripts was studie d by northern blot analysis using total RNA isolated from several porc ine tissues taken at three different ages (day 1, after 5 weeks and at 100 kg weight). Hybridisation were carried out with two different pro bes, one binding to both PPAR gamma transcripts and the other being PP AR gamma 2 specific. Strongest hybridisation signals with the PPAR gam ma probe binding both variants were detected in adipose tissues and sp leen at all three ages, whereas only faint or no signals were detected in other tissues. The tissue distribution pattern of PPAR gamma 1 and gamma 2 suggests a modulation of tissue distribution for the two tran scripts and obvious age and breed differences in gene expression in sw ine. (C) 1998 Academic Press.