Toxicity tests on the effect of tobacco dust against brackishwater pon
d snails (Cerithidea cingulata Gmelin) of various stages or size range
s were conducted under controlled laboratory conditions. The 72-h LC50
for the juveniles, sub-adult and adult snails are 30, 87 and 166 kg h
a(-1), respectively, while the 72-h LC99 are 290, 522 and 712 kg ha(-1
), respectively. The nicotine content of the tobacco dust used was 2.8
%. Lethal concentration increased as the life stages or size of the sn
ail increased. Molluscicidal activity also progressively increased wit
h time of exposure. The nicotine content and toxicity of different typ
es of tobacco [flue-cured (neutral), flue-cured (full-flavor), burley
and batek (full-flavor)] were determined on adult snails. Lethal conce
ntrations (LC50) and (LC99) decreased as nicotine content of the tobac
co dust increased indicating a higher molluscicidal activity. The effe
ctive application rate is affected by the nicotine content of the toba
cco dust. An equivalent nicotine concentration of ca 24 kg ha(-1) corr
esponded with the 72-h LC99 under laboratory conditions. Seven-day bio
assay experiments on the effect of tobacco dust on two size groups of
milkfish juveniles (0.5 and 2-3 g) showed no mortalities up to the con
centration lethal to the pond snails. (C) 1998 Elsevier Science Ltd. A
ll rights reserved.