European hedgehog populations belonging to Erinaceus europaeus and E.
concolor have been investigated by mitochondrial DNA analysis. A 383 b
p fragment of the cytochrome b gene has been sequenced and maximum par
simony and neighbour-joining trees of Tamura-Nei genetic distance valu
es have been constructed. Similar topologies have been produced by bot
h methods, showing a deep divergence between E. europaeus and E. conco
lor and a further subdivision of each species into a western and an ea
stern clade. A comparison with previously published allozyme data is m
ade, and concordant and discordant patterns are discussed. The influen
ce of Pleistocene glaciations on the observed pattern of divergence is
inferred.