Kt. Kruseelliott et al., LOW-MOLECULAR-WEIGHT HEPARIN ALTERS PORCINE NEUTROPHIL RESPONSES TO PLATELET-ACTIVATING-FACTOR, Shock, 10(3), 1998, pp. 198-202
Citations number
26
Categorie Soggetti
Peripheal Vascular Diseas","Emergency Medicine & Critical Care",Hematology,Surgery
Because platelet-activating factor (PAF) is an important mediator of i
nflammation and heparin has anti-inflammatory effects, we hypothesized
that low molecular weight heparin (LMWH) would inhibit PAF-induced ac
tivation and chemotaxis in porcine neutrophils. Citrated blood was obt
ained from pentobarbital-anesthetized pigs, and neutrophils were isola
ted over a 55%/65% Percoll gradient. The effect of LMWH on basal phorb
ol myristate acetate (PMA)-induced superoxide (SO) release, as well as
its effect on PAF priming for PMA-induced SO release, were investigat
ed. Additionally, the effect of LMWH on PAF-induced chemotaxis of neut
rophils across transwell membranes was evaluated. Baseline SO release
in response to PMA was .351 +/- .046 nmol/10(6) cells/min, and this wa
s decreased to .289 +/- .034 nmol/10(6) cells/min by pretreatment with
50 U/mL LMWH. PMA-induced SO production was increased by .240 +/- .04
2 nmol/10(6) cells/min when cells were primed with 10 mu M PAF. This p
riming effect of PAF was reduced significantly by pretreatment of neut
rophils with LMWH at 10 and 50 U/mL. Chemotaxis of neutrophils in resp
onse to 100 mu M PAF was significantly decreased to 70.02 +/- 6.4% (n
= 8) of the control response by pretreatment of cells with 50 U/mL LMW
H. We conclude that LMWH has anti-inflammatory effects on porcine neut
rophils, which includes attenuation of cell activation and chemotaxis
in response to the lipid-derived inflammatory mediator, PAF.