EFFECTS OF GLYCOSYLATION OF THE RESIDUE AT POSITION-14 IN-OVINE ANGIOTENSINOGEN ON THE HUMAN RENIN REACTION

Authors
INUI Y ORIHASHI T NAKAGAWA T EBIHARA A SUZUKI F NAKAMURA Y
Citation
Y. Inui et al., EFFECTS OF GLYCOSYLATION OF THE RESIDUE AT POSITION-14 IN-OVINE ANGIOTENSINOGEN ON THE HUMAN RENIN REACTION, Bioscience, biotechnology, and biochemistry, 62(8), 1998, pp. 1612-1614
Citations number
12
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
62
Issue
8
Year of publication
1998
Pages
1612 - 1614
Database
ISI
SICI code
0916-8451(1998)62:8<1612:EOGOTR>2.0.ZU;2-6
Abstract
A mutant angiotensinogen, S14N, in which Ser(14) of ovine angiotensino gen was replaced by Asn to form a N-glycosylation site, was produced i n CHO cells. The molecular weight was about 3,000 larger than that of wild-type ovine angiotensinogen, indicating that S14N angiotensinogen was glycosylated at Asn(14). In the reaction with human renin, the K-m of mutant angiotensinogen was 3 times increased, but the V-max was no t affected by the mutation.