COMPARATIVE MOLECULAR EPIDEMIOLOGIC INVESTIGATION ON DIFFERENT BOVINEHERPES VIRUSES

Eight Bulgarian bovine herpes viruses, two Hungarian herpes viruses 1A , 3A, calves isolate named Mramor, buffalo isolate 723 and two referen ts BHV 1 strains were investigated by restrictase fragment pattern ana lysis. Migration profile of viral DNA by using different restrictase e nzymes Hpa I, BamH I and Hind III were compared. Clearly differences a mong two Hungarian strains, calves isolate Mramor, buffalo isolate 723 and 8 Bulgarian and two referents BHV 1 strain was observed. The stra in Sartze was determined as a genital type BHV 1, whereas Ozet, Tch.vo da, Slivnitza, B. Budinov, Ptcelarovo, Vrana and Podgumer as a respira tory type. Hungarian strains 1A, 3A, calves isolate Mramor and buffalo isolate 723 had similar migration profile as swine herpes viruses. Hy bridisation between the K 22 fragment and 8 bovine herpes viruses afte r Southern blotting were observed. That is evidence for genetic relati on of these strains. Such hybridisation with Hungarian 1A, 3A, Mramor and buffaloes 723 strains were not observed. This fact allowed us to c onclude that these strains are genetically different from BHV 1. (C) 1 998 Elsevier Science Ltd. All rights reserved.