NEW TRIMETHOPRIM-RESISTANT DIHYDROFOLATE-REDUCTASE CASSETTE, DFRXV, INSERTED IN A CLASS-1 INTEGRON

Authors
ADRIAN PV DUPLESSIS M KLUGMAN KP AMYES SGB
Citation
Pv. Adrian et al., NEW TRIMETHOPRIM-RESISTANT DIHYDROFOLATE-REDUCTASE CASSETTE, DFRXV, INSERTED IN A CLASS-1 INTEGRON, Antimicrobial agents and chemotherapy, 42(9), 1998, pp. 2221-2224
Citations number
31
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
42
Issue
9
Year of publication
1998
Pages
2221 - 2224
Database
ISI
SICI code
0066-4804(1998)42:9<2221:NTDCDI>2.0.ZU;2-7
Abstract
The nucleotide sequence of a plasmid-borne trimethoprim resistance gen e from a commensal fecal Escherichia coli isolate revealed a new dihyd rofolate reductase gene, dfrXV, which occurred as a gene cassette inte grated in a site-specific manner in a class 1 integron. The new gene s hows 84% nucleotide identity and the predicted protein shows 90% amino acid identity with dfrI and DHFR type I, respectively. Genes for spec tinomycin resistance, aadA1 [ant (3 '')-Ia], and sulfonamide resistanc e, sul1, were located downstream of dfrXV in a manner identical to tha t in pLMO229.