THE NIR-Q GENE, WHICH IS REQUIRED FOR DENTRIFICATION OF PSEUDOMONAS-AERUGINOSA, CAN ACTIVATE THE RUBISCO FROM PSEUDOMONAS-HYDROGENOTHERMOPHILA

Two putative ATP-binding proteins encoded in the gene cluster for the Calvin cycle of Pseudomonas hydrogenothermophila (cbbQ) and for the de nitrification of Pseudomonas aeruginosa (nirQ) have been found to be s imilar. The cbbQ gene has been shown to activate the RubisCO from P. h ydrogenothermophila in E. coli. The nirQ was functionally substituted for cbbQ. The nirQ gene restored the anaerobic growth and the NOR acti vity of the nirQOP mutant of P. aeruginosa, while the cbbQ gene did no t. (C) 1998 Elsevier Science B.V. All rights reserved.