EFFECT OF PYRIDOXINE AND INSULIN ADMINISTRATION ON BRAIN GLUTAMATE-DEHYDROGENASE ACTIVITY AND BLOOD-GLUCOSE CONTROL IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

Blood glucose level and kinetic parameters of glutamate dehydrogenase (GDH) were measured in the cerebellum, brain stem and cerebral cortex of control, insulin treated, pyridoxine treated, pyridoxine and insuli n treated and untreated streptozotocin-diabetic rats. The combined adm inistration of insulin and pyridoxine was found to be better in contro lling the hyperglycaemia. Insulin with pyridoxine treatment brought ba ck the increased maximal velocity of GDH during diabetes to control st ate. Also, there was an increase in Michaelis-Menten constant. These r esults suggest that pyridoxine and insulin together serve a better con trol for diabetes. (C) 1998 Elsevier Science B.V. All rights reserved.