SOMATIC EMBRYOGENESIS AND PLANT-REGENERATION IN AZADIRACHTA-INDICA A JUSS

Authors
SHRIKHANDE M THENGANE SR MASCARENHAS AF
Citation
M. Shrikhande et al., SOMATIC EMBRYOGENESIS AND PLANT-REGENERATION IN AZADIRACHTA-INDICA A JUSS, In vitro cellular & developmental biology. Plant, 29P(1), 1993, pp. 38-42
Citations number
20
Categorie Soggetti
Developmental Biology","Cytology & Histology","Plant Sciences
Journal title
In vitro cellular & developmental biology. Plant → ACNP
ISSN journal
10545476
Volume
29P
Issue
1
Year of publication
1993
Pages
38 - 42
Database
ISI
SICI code
1054-5476(1993)29P:1<38:SEAPIA>2.0.ZU;2-8
Abstract
Media for induction of somatic embryogenesis from immature cotyledonar y tissues of Azadirachta indica (Neem) were determined. Callus was ini tiated on Murashige and Skoog medium supplemented with 0.5 mg . liter- 1 of indol-3 acetic acid, 1.0 mg . liter-1 of 6-benzyl amino purine, a nd 1000 mg . liter-1 of casein hydrolysate. Effect of kinetin was also studied for embryo induction. Carbohydrate source in the form of sucr ose and glucose alone and in combination was tested for embryogenic ef ficiency. Seventy percent embryos showed germination. Healthy plants w ere potted in sand and soil. Histologic studies confirmed indirect som atic embryogenesis.