LIPOTEICHOIC ACID INDUCES PRETERM DELIVERY IN MICE

The purpose of this study was to determine whether or not lipoteichoic acid (LTA) could induce preterm delivery in mice. On days 15 and 17 o f pregnancy, female C3H/HeN mice impregnated by male B6D2F(1) mice wer e given intraperitoneal injections of LTA (12.5-75 mg/kg, single dose or repeated doses at a 3-h interval). We examined the changes in cervi x, placental trophoblasts, and plasma and amniotic fluid concentration s of interleukin-l alpha (IL-1 alpha), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) after dosing with LTA. In addition, the effect of LTA on the contraction of isolated uterine muscle from pregnant mice was also measured. The incidence of preterm delivery was highest (100%), when the pregnant animals were treated with 75 mg/kg LTA twice on day 15 of pregnancy or with 25 mg/kg LTA twice on day 17 of pregnancy. LTA-accelerated cervical ripening and placental abruptio n preceding the onset of preterm delivery, as well as increased plasma and amniotic fluid concentrations of IL-1 alpha, IL-6, and TNF-alpha. Also, LTA increased contraction of uterine muscle strips. In conclusi on, LTA induced preterm delivery in mice in the same manner as lipopol ysaccharide (LPS), but the effective dose of LTA was larger than that of LPS. (C) 1998 Elsevier Science Inc.