Ee. Zijlstra et al., RK39 ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DIAGNOSIS OF LEISHMANIA-DONOVANI INFECTION, Clinical and diagnostic laboratory immunology, 5(5), 1998, pp. 717-720
The rK39 enzyme-linked immunosorbent assay (ELISA) was compared with t
he direct agglutination test (DAT) for Leishmania donovani infection i
n the Sudan. rK39 ELISA proved more sensitive than DAT in diagnosis of
kala-azar (93 and 80%, respectively); both tests may remain positive
up to 24 months after treatment. For patients with post-kala-azar derm
al leishmaniasis and individuals with subclinical infection, rK39 ELIS
A performed as well as DAT but could detect infection 6 months earlier
in similar to 40% of patients. Conversion in DAT and rK39 ELISA also
occurred in leishmanin skin test (LST)-positive individuals, suggestin
g active parasite replication (rK39 is an amastigote antigen) in these
presumably immune individuals. In contrast to DAT, rK39 ELISA also de
tected infection in randomly selected LST-positive individuals (in fou
r of six) and endemicity (LST-negative) controls (in one of five). rK3
9 ELISA appears more sensitive than DAT and may prove an important too
l in epidemiological studies.