Resistance to imazethapyr was identified in a population of common sun
flower that had been treated with imazethapyr for seven consecutive ye
ars. The imazethapyr resistant biotype of common sunflower was approxi
mately 170 times more resistant to imazethapyr than the susceptible bi
otype based on the rate required for 25% control. Resistance was due t
o altered acetolactate synthase (ALS) that is less sensitive to imazet
hapyr. The imazethapyr concentration required to inhibit in vitro ALS
activity by 25% was 210-fold higher in the resistant biotype than in c
he susceptible biotype. Differences in absorption, translocation, and
metabolism of imazethapyr in common sunflower biotypes were not suffic
ient to explain the resistance to imazethapyr.