ANALYSIS OF DELETED VARIANT OF HEPATOCYTE GROWTH-FACTOR BY ALANINE SCANNING MUTAGENESIS - IDENTIFICATION OF RESIDUES ESSENTIAL FOR ITS BIOLOGICAL FUNCTION AND GENERATION OF MUTANTS WITH ENHANCED MITOGENIC ACTIVITY ON RAT HEPATOCYTES
M. Kinosaki et al., ANALYSIS OF DELETED VARIANT OF HEPATOCYTE GROWTH-FACTOR BY ALANINE SCANNING MUTAGENESIS - IDENTIFICATION OF RESIDUES ESSENTIAL FOR ITS BIOLOGICAL FUNCTION AND GENERATION OF MUTANTS WITH ENHANCED MITOGENIC ACTIVITY ON RAT HEPATOCYTES, FEBS letters, 434(1-2), 1998, pp. 165-170
To understand the structure-function relationship of hepatocyte growth
factor (HGF) in more detail, we analyzed one of the other forms of HG
F, deleted variant of HGF (dHGF), by alanine scanning mutagenesis, We
show here that there are at least four sites important for dHGF to sti
mulate DNA synthesis in cultured adult rat hepatocytes, and that the r
esidues of HGF essential for exerting its biological activity are not
identical to those of dHGF. In addition, two mutants showed a decrease
(approximately three-fold) in EC50 compared with wild-type dHGF in an
assay of mitogenic activity on rat hepatocytes, (C) 1998 Federation o
f European Biochemical Societies.