ANALYSIS OF DELETED VARIANT OF HEPATOCYTE GROWTH-FACTOR BY ALANINE SCANNING MUTAGENESIS - IDENTIFICATION OF RESIDUES ESSENTIAL FOR ITS BIOLOGICAL FUNCTION AND GENERATION OF MUTANTS WITH ENHANCED MITOGENIC ACTIVITY ON RAT HEPATOCYTES

Authors
KINOSAKI M YAMAGUCHI K MURAKAMI A MORINAGA T UEDA M HIGASHIO K
Citation
M. Kinosaki et al., ANALYSIS OF DELETED VARIANT OF HEPATOCYTE GROWTH-FACTOR BY ALANINE SCANNING MUTAGENESIS - IDENTIFICATION OF RESIDUES ESSENTIAL FOR ITS BIOLOGICAL FUNCTION AND GENERATION OF MUTANTS WITH ENHANCED MITOGENIC ACTIVITY ON RAT HEPATOCYTES, FEBS letters, 434(1-2), 1998, pp. 165-170
Citations number
41
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
434
Issue
1-2
Year of publication
1998
Pages
165 - 170
Database
ISI
SICI code
0014-5793(1998)434:1-2<165:AODVOH>2.0.ZU;2-Z
Abstract
To understand the structure-function relationship of hepatocyte growth factor (HGF) in more detail, we analyzed one of the other forms of HG F, deleted variant of HGF (dHGF), by alanine scanning mutagenesis, We show here that there are at least four sites important for dHGF to sti mulate DNA synthesis in cultured adult rat hepatocytes, and that the r esidues of HGF essential for exerting its biological activity are not identical to those of dHGF. In addition, two mutants showed a decrease (approximately three-fold) in EC50 compared with wild-type dHGF in an assay of mitogenic activity on rat hepatocytes, (C) 1998 Federation o f European Biochemical Societies.