DENSITY-DEPENDENT AND PROLIFERATION-STATUS-DEPENDENT EXPRESSION OF T-CADHERIN, A NOVEL LIPOPROTEIN-BINDING GLYCOPROTEIN - A FUNCTION IN NEGATIVE REGULATION OF SMOOTH-MUSCLE CELL-GROWTH
Ys. Kuzmenko et al., DENSITY-DEPENDENT AND PROLIFERATION-STATUS-DEPENDENT EXPRESSION OF T-CADHERIN, A NOVEL LIPOPROTEIN-BINDING GLYCOPROTEIN - A FUNCTION IN NEGATIVE REGULATION OF SMOOTH-MUSCLE CELL-GROWTH, FEBS letters, 434(1-2), 1998, pp. 183-187
The atypical low density lipoprotein (LDL) binding proteins (M-r 105 a
nd 130 kDa; p105 and p130) in human aortic medial membranes and cultur
ed human and rat aortic smooth muscle cells (SMC) have recently been i
dentified as the cell adhesion glycoprotein T-cadherin, Although cadhe
rins are generally recognized to be important regulators of morphogene
sis, the function of T-cadherin in the vasculature is poorly understoo
d. This study has examined the relationship between expression of T-ca
dherin and the density and proliferation status of SMC, T-cadherin (p1
05 and p130) levels in SMC lysates mere measured on Western blots usin
g ligand-binding techniques. T-cadherin expression was dependent upon
cell density, and maximal levels mere achieved at confluency. T-cadher
in levels mere reversibly modulated by switching cultures between seru
m-free (upmodulation) and serum-containing (downmodulation) conditions
. Platelet-derived growth factor (PDGF)-BB, epidermal growth factor (E
GF) or insulin-like growth factor (IGF) elicited a dose- and time-depe
ndent downmodulation that was reversible after transfer of SMC to grow
th factor-free medium. Our results support the hypothesis that T-cadhe
rin may function as a negative determinant of cell growth, (C) 1998 Fe
deration of European Biochemical Societies.