ASSIGNMENT OF THE MOUSE AND COW CXC CHEMOKINE GENES

Gene specific PCR primers were constructed for five mouse and three bo vine CXC chemokine genes. The mouse genes were assigned using SSCP ana lyses of the Jackson BSS backcross panel to two groups on chromosome 5 . One group containing Gro1 and Mip2 cosegregated with reference marke rs Alb1 and Btc, and was positioned 2.2 cM proximal to a group compris ing Ifi10, Mig, and Scyb5. The bovine genes IL8, GRO1, and GRO3, mappe d using bovine x hamster somatic cell hybrids, were all found to be lo cated on chromosome 6. The locations of these genes in these two anima l species are consistent with the positions in humans (4q13-->q21), an d previous syntenic relationships among these three mammals.