MONOCLONAL-ANTIBODIES DIRECTED AGAINST LIPOPOLYSACCHARIDE OF LEGIONELLA-PNEUMOPHILA SEROGROUP-1

Authors
BARANOV AM VETCHININ SS BARANOVA EV BIKETOV SF SVETOCH EA
Citation
Am. Baranov et al., MONOCLONAL-ANTIBODIES DIRECTED AGAINST LIPOPOLYSACCHARIDE OF LEGIONELLA-PNEUMOPHILA SEROGROUP-1, Biochemistry and molecular biology international, 45(6), 1998, pp. 1163-1168
Citations number
16
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
45
Issue
6
Year of publication
1998
Pages
1163 - 1168
Database
ISI
SICI code
1039-9712(1998)45:6<1163:MDALOL>2.0.ZU;2-8
Abstract
The monoclonal antibodies (MAbs) against lipopolysaccharide of virulen t strain of Legionella pneumophila serogroup 1 were produced. Three mo st productive hybrid clones (5F4, 5F10 and 2C9) were selected from fus ions of mouse myeloma cells with spleen cells from BALB/c mice, immuni zed with bacterial outer membrane antigens. All generated clones were IgG-secreting. The MAbs had narrow strain specificity and showed no cr oss-reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs coul d be used for diagnostic purposes.