A COMPARATIVE-STUDY BY A SINGLE CHROMATOGRAPHIC PROCEDURE OF GLYCOLYTIC REGULATORY KINASE ISOZYMES IN RAT ERYTHROID-CELLS AS A FUNCTION OF DIFFERENTIATION-MATURATION PROCESS

The isozymes of three glycolytic regulatory kinases: hexokinase, phosp hofructokinase and pyruvate kinase are fractionated by a single ion ex change chromatographic procedure on DEAE-cellulose. Enriched-erythrobl ast bone marrow cells showed two heterogeneous peaks, each consisting of two overlapping peaks: one major and one minor peak, but only two i sozymes were observed in reticulocytes and erythrocytes. Phosphofructo kinase showed multiple isozymic form in the three cell populations, bu t while in erythroblasts the main one eluted in the last fractions, in reticulocytes and erythrocytes it eluted in the early fractions. Pyru vate kinase showed a main early activity peak with a shoulder in eryth roblasts, reticulocytes and erythrocytes but the response to the allos teric effecters (fructose-1,6-bisphosphate and ATP) suggests the prese nce of different pyruvate kinase isozymes in reticulocytes and erythro cytes.