INTEGRATED MAP OF AFLP, SSLP AND RFLP MARKERS USING A RECOMBINANT INBRED POPULATION OF RICE (ORYZA-SATIVA L.)

A molecular map of rice consisting of 231 amplified fragment length po lymorphisms (AFLPs), 212 restriction fragment length polymorphisms (RF LPs), 86 simple-sequence length polymorphisms (SSLPs), five isozyme lo ci, and two morphological mutant loci [phenol staining of grain (Ph), semi-dwarf habit (sd-1)] has been constructed using an F-11 recombinan t inbred (RI) population. The mapping population consisted of 164 RI l ines and was developed via single-seed descent from an intercross betw een the genetically divergent parents Milyang 23 (M) (tongil type) and Gihobyeo (G) (japonica type). A subset of previously mapped RFLP and SSLP markers were used to construct the map framework. The AFLP marker s were derived from ten EcoRI(+2) and MseI(+3) primer combinations. Al l marker types were well distributed throughout the 12 chromosomes. Th e integrated map covered 1814 cM, with an average interval size of 3.4 cM. The MG map is a cornerstone of the Korean Rice Genome Research Pr ogram (KRGRP) and is being continuously refined through the addition o f partially sequenced cDNA markers derived from an immature-seed cDNA library developed in Korea, and microsatellite markers developed at Co rnell. The population is also being used for quantitative trait locus (QTL) analysis and as the basis for marker-assisted variety developmen t.