Yb. Blume et al., ALTERATION OF BETA-TUBULIN IN NICOTIANA-PLUMBAGINIFOLIA CONFERS RESISTANCE TO AMIPROPHOS-METHYL, Theoretical and Applied Genetics, 97(3), 1998, pp. 464-472
A Nicotiana plumbaginifolia plant (apm5(r)) resistant to amiprophos-me
thyl (APM), a phosphoroamide herbicide, was isolated from protoplasts
prepared from leaves of haploid plants. Genetic analysis revealed that
the resistance is coded for by a dominant nuclear mutation and is ass
ociated with the increased stability of cortical microtubules. Two-dim
ensional polyacrylamide-gel electrophoresis, combined with immunoblott
ing using anti-tubulin monoclonal antibodies, showed that part of the
beta-tubulin in the resistant plant possessed lower isoelectric points
than the beta-tubulin of susceptible wild-type plants. These results
provide evidence that the resistance to APM is associated with a mutat
ion in a beta-tubulin gene. The APM-resistant line showed cross-resist
ance to trifluralin, a dinitroaniline herbicide, suggesting a common m
echanism of resistance between these two classes of herbicides.