CORRELATION OF P-32 POSTLABELING-DETECTION OF DNA-ADDUCTS IN MOUSE SKIN IN-VIVO WITH THE POLYCYCLIC AROMATIC COMPOUND CONTENT AND MUTAGENICITY IN SALMONELLA-TYPHIMURIUM OF A RANGE OF OIL PRODUCTS

The in vivo genotoxic activities in mouse skin of the dimethyl sulphox ide (DMSO) extracts of a range of oil products [residual aromatic extr act: untreated heavy paraffinic distillate aromatic extract; mildly re fined light naphthenic base oil; bitumen (vacuum residue); high viscos ity index base oil obtained by catalytic hydrogenation] were evaluated by P-32-postlabelling DNA analysis. The results of quantitative P-32- postlabelling analyses of epidermal DNA from mice treated with the DMS O extracts showed linear relationships with the total polycyclic aroma tic compound (PAC) contents, determined by the Institute of Petroleum method IP 346 and also the 3-6 ring PAC contents, measured by on-line liquid-liquid extraction using flow injection analysis. The P-32-postl abelling data also showed a linear relationship with the mutagenicity indices of these oil products determined in S. typhimurium TA98 using the modified Ames Salmonella microsome test. The in vivo genotoxicity of the DMSO extracts from the oil products was low, judged by P-32-pos tlabelling analysis of DNA adducts measured in epidermal DNA of treate d mouse skin, and ranging from 2 to 723 attomole/mu g DNA per mg oil p roduct. The in vivo P-32-postlabelling data from this study are consis tent with these materials expressing low genotoxicity in mouse skin in vivo. The DMSO extraction procedure coupled with P-32-postlabelling D NA analysis is useful for ranking the relative genotoxic potency in vi vo of a wide range of oil products. In general the trend observed is s imilar to rankings based on physicochemical measurements of total PAC contents or 3-6 ring PAC contents of the oil products.