EXPERIMENTAL-MODELS, PROTOCOLS, AND REFERENCE VALUES FOR EVALUATION OF IODINATED ANALOGS OF GLUCOSE

For an iodinated analogue of glucose to be useful for evaluating gluco se uptake using single-photon emission computed tomography (SPECT), ii must enter the cell via the same transporter as glucose and accumulat e within the cell without being degraded. The biological behavior of t he iodinated tracer must therefore be similar to that of 2-deoxy-D-[1- C-14]-glucose (2-DG). In the present study, four experimental models ( biodistribution in mouse, isolated rat heart, human erythrocytes in su spension and cultured neonatal rat cardiomyocytes) have been chosen an d protocols have been set up which allow for the examination of small quantities of iodinated analogues of glucose. The uptakes of 2-DG and of L-[1-C-14]-glucose have been measured in these models to establish reference values which will be compared with uptake values for iodinat ed analogues of glucose.