G. Segarra et al., ROLE OF VASOPRESSIN ON ADRENERGIC NEUROTRANSMISSION IN HUMAN PENILE BLOOD-VESSELS, The Journal of pharmacology and experimental therapeutics, 286(3), 1998, pp. 1315-1320
We have used in vitro preparations of human penile dorsal artery and d
eep dorsal vein from 20 multiorgan donors to investigate whether subpr
essor concentrations of vasopressin facilitate noradrenergic transmiss
ion in penile blood vessels. Vasopressin constricted penile dorsal art
eries (pD(2),, 9.38 +/- 0.18) and deep dorsal veins (pD(2),, 9.40 +/-
0.14) by activating V-1, receptors. Vasopressin (10(-11) and 3 x 10(-1
1) M) caused concentration-dependent potentiation of the contractions
elicited by electrical stimulation (15 V, 0.5-2 Hz, 0.2 msec duration
for 15 sec) and produced leftward shifts of the concentration-response
curve for norepinephrine. The V-1 receptor antagonist d(CH2)(5)Tyr(Me
)AVP (3 x 10(-9)-10(-7) M) induced concentration-dependent inhibitions
of potentiation caused by vasopressin. In contrast, the V-2, receptor
antagonist [d(CH2)5,D-IIe(2),IIe(4),Arg(8)]-vasopressin (10(-8)-10(-7
) M) did not prevent the potentiation induced by vasopressin. The resu
lts demonstrate that vasopressin exerts powerful constrictor action in
human penile arteries and veins by direct stimulation of V-1, recepto
rs. in addition, vasopressin strongly potentiates the contractions to
norepinephrine and stimulation of perivascular adrenergic nerves. Cons
equently, the direct contractile effects of vasopressin together with
its amplifying effects on adrenergic-mediated constriction should be t
aken into consideration in the overall regulation of penile erection a
nd in those states characterized by increased plasma vasopressin level
s.