SERS STUDIES ON A NOVEL COPPER PORPHYRIN AND ITS COMPLEX WITH DNA

The SERS spectra of topper (II) complex of meso-tetris (4-N-cyano meth yl pyridyl) porphyrin (Cu(II)NACN) and the complexes with the calf thy mus double-stranded and denatured DNA on silver sols obtained by reduc ing AgNO3 with citrate and the effect of pH and the concentration of C u(II)NACN have been investigated. It was pointed out that, pH=5 simila r to 6, the concentration of Cu(II)NACN ca 1x10(-5) mol/L are better f or SERS. Cu(II)NACN acts on Ag sols through the pyridyl N and the porp hyrin core together, and is absorbed on Ag sols in the slope-on form w hich is not influenced by adding the double stranded and the denatured DNA. In the system of Ag sols, Cu(II)NACN acts on the double stranded DNA at phosphate by electrostatic force, but maybe part-intercalates the single stranded of the denatured DNA accompanied with the electros tatic force similar to the double stranded DNA.