Major problems in oil and gas operations result from the biogenic form
ation of H2S in the reservoir. The presence of H2S results in increase
d corrosion, iron sulfide formation, higher operating costs, reduced r
evenue, and constitutes a serious environmental and health hazard. A n
ew technology that removes and prevents the formation of biogenic H2S
has been developed. The technology is based on the addition of low con
centrations of a water-soluble nutrient solution, which selectively st
imulates the growth of an indigenous microbial population, thereby inh
ibiting the detrimental sulfate-reducing bacteria (SRB) population, wh
ich causes the generation of H2S. This deliberate and controlled modif
ication of the microflora and reservoir ecology has been termed biocom
petitive exclusion. This directed alteration of the microbial ecology
is achieved by the introduction of a nutrient formulation that favorab
ly influences the dynamics of the reservoir microflora. The treatment
system does not require the addition of microbial cultures. Periodic w
ell treatments have been shown to dramatically reduce or completely el
iminate sulfide levels and establish control of the system in diverse
field demonstrations supported by the U.S. Dept. of Energy. Field data
in a variety of reservoirs will be presented that demonstrate the eff
ectiveness of this new approach to treating and remediating wells with
high levels of H2S. The versatility and low cost of this novel techno
logy offers the petroleum industry a practical and cost effective meth
odology for the control of H2S in oil and gas wells.