TIGHT-BINDING STREPTAVIDIN LIGANDS FROM A CYCLIC PEPTIDE LIBRARY

Authors
ZANG X YU ZG CHU YH
Citation
X. Zang et al., TIGHT-BINDING STREPTAVIDIN LIGANDS FROM A CYCLIC PEPTIDE LIBRARY, Bioorganic & medicinal chemistry letters, 8(17), 1998, pp. 2327-2332
Citations number
44
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
8
Issue
17
Year of publication
1998
Pages
2327 - 2332
Database
ISI
SICI code
0960-894X(1998)8:17<2327:TSLFAC>2.0.ZU;2-K
Abstract
During the screening of a soluble library of cyclo(AXXXXXAE)K-CONH2, a cyclic peptide cyclo(AHPQFPAE)K-CONH2 was identified as a tight-bindi ng ligand (IC50 = 128 nM) and found to bind 1000-fold more tightly tha n its linear peptide to streptavidin. The results of this study sugges t that library screening of conformationally constrained cyclic peptid es can be an effective means for the discovery of high affinity ligand s. (C) 1998 Elsevier Science Ltd. All rights reserved.