CUT AND COMBINE - AN EASY MEMBRANE-SUPPORTED COMBINATORIAL SYNTHESIS TECHNIQUE

A combinatorial synthesis process involving sequential cycles of cutti ng a membrane support into pieces and combining these into groups and subjecting the groups to simultaneous solid-phase chemical reactions i s demonstrated by the rapid assembly of four hundred N-terminally biot inylated, soluble, octameric peptide pools. Index patterns printed ont o the synthesis membrane allowed a direct identification of the compou nds. These were used to study protein kinase substrate selection in a parallel microplate adapted P-32-phosphorylation assay with subsequent spotting on a biotin-capture membrane, (C) 1998 Elsevier Science Ltd. Ali rights reserved.