INFLUENCE OF 2-ACETYLAMINOFLUORENE (2-AAF) ON BIOTRANSFORMATION AND LIPID-PEROXIDATION IN SALIVARY-GLANDS AND LIVER FROM MALE RATS

Salivary glands proved to be active in biotransformation. In microsome s of rat salivary glands 7-ethoxyresorufin O-deethylation (EROD) and 7 -pentoxyresorufin O-depentylation (PEROD) were detectable, but with mu ch lower activities than in the liver. Beside the well-known induction of EROD or PEROD in the liver by beta-naphthoflavone (BNF) or phenoba rbital (PB), respectively, a marked rise in EROD rate of salivary glan ds was observed after BNF treatment. Administration of 2-AAF caused an increase in EROD rates in liver microsomes, but a decrease in microso mes of salivary glands. This decrease in EROD rate was accompanied by selective cytotoxic damages in the convoluted granulated tubules of th e submandibular glands. No cytotoxic damage occurred in the submandibu lar glands after a combined administration of the inducer BNF and 2-AA F. This indicates relations between these toxic effects of 2-AAF and c hanges of 2-AAF-metabolism in BNF-induced rats, maybe in the liver and /or in the submandibular glands themselves.