GENETIC-ANALYSIS OF THE YEAST SACCHAROMYCES-CASTELLII, SACCHAROMYCES-EXIGUUS, AND SACCHAROMYCES-MARTINIAE

Genetic lines of three biological yeast species of the Saccharomyces s ensu lato group-S. castellii, S. exiguus, and S. martiniae-were create d. Homo- and heterothallic monosporic fertile strains were marked by d ifferent auxotrophic mutations. Conditions for the hybridization of th e Saccharomyces sensu jato yeast were established. The mating of spore s or haploid cells of auxotrophic complementary mutants was performed on the complete YPD medium. Prototrophic diploid hybrids were selected on a minimal medium. In S. castellii, the hybridization of diploid ce lls was demonstrated. All three species studied were suitable for tetr ad analysis.