M. Rousset et al., HETEROLOGOUS EXPRESSION OF THE DESULFOVIBRIO-GIGAS [NIFE] HYDROGENASEIN DESULFOVIBRIO FRUCTOSOVORANS MR400, Journal of bacteriology, 180(18), 1998, pp. 4982-4986
The ability of Desulfovibrio fructosovorans MR400 Delta hynABC to expr
ess the heterologous cloned [NiFe] hydrogenase of Desulfovibrio gigas
was investigated. The [NiFe] hydrogenase operon from D. gigas, hynABCD
, was cloned, sequenced, and introduced into D, fructosovorans MR400.
A portion of the recombinant heterologous [NiFe] hydrogenase was total
ly matured, exhibiting catalytic and spectroscopic properties identica
l to those of the native D. gigas protein. A chimeric operon containin
g hynAB from D. gigas and hynC from D. fructosovorans placed under the
control of the D. fructosovorans hynAp promoter was constructed and e
xpressed in D. fructosovorans MR400. Under these conditions, the same
level of activity was obtained as with the D, gigas hydrogenase operon
.