A. Rosner et al., DIFFERENTIATION AMONG ISOLATES OF PRUNUS NECROTIC RINGSPOT VIRUS BY TRANSCRIPT CONFORMATION POLYMORPHISM, Journal of virological methods, 74(1), 1998, pp. 109-115
Citations number
18
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
A method based on differences in electrophoretic mobility of RNA trans
cripts made from polymerase chain reaction (PCR) products was used for
differentiation among virus isolates. A T7 RNA polymerase promoter wa
s attached to amplified prunus necrotic ringspot virus (PNRSV) sequenc
es by PCR. The PCR products then served as a template for transcriptio
n. Single-stranded transcripts originated from different PNRSV isolate
s varied in electrophoretic mobility in polyacrylamide gels, presumabl
y because of transcript conformation polymorphism (TCP). This procedur
e was applied for the differentiation of PNRSV isolates. (C) 1998 Else
vier Science B.V. All rights reserved.