SYNTHESIS, BIOPHYSICAL PROPERTIES, AND NUCLEASE RESISTANCE PROPERTIESOF MIRED BACKBONE OLIGODEOXYNUCLEOTIDES CONTAINING CATIONIC INTERNUCLEOSIDE GUANIDINIUM LINKAGES - DEOXYNUCLEIC GUANIDINE DNA CHIMERAS/

Authors
BARAWKAR DA BRUICE TC
Citation
Da. Barawkar et Tc. Bruice, SYNTHESIS, BIOPHYSICAL PROPERTIES, AND NUCLEASE RESISTANCE PROPERTIESOF MIRED BACKBONE OLIGODEOXYNUCLEOTIDES CONTAINING CATIONIC INTERNUCLEOSIDE GUANIDINIUM LINKAGES - DEOXYNUCLEIC GUANIDINE DNA CHIMERAS/, Proceedings of the National Academy of Sciences of the United Statesof America, 95(19), 1998, pp. 11047-11052
Citations number
28
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
95
Issue
19
Year of publication
1998
Pages
11047 - 11052
Database
ISI
SICI code
0027-8424(1998)95:19<11047:SBPANR>2.0.ZU;2-P
Abstract
The synthesis of mixed backbone oligodeoxynucleotides (18-mers) consis ting of positively charged guanidinium linkages along with negatively charged phosphodiester linkages is carried out. The use of a base labi le-protecting group for guanidinium linkage offers a synthetic strateg y similar to standard oligonucleotide synthesis. The nuclease resistan ce of the oligodeoxyribonucleotides capped with guanidinium linkages a t 5' and 3' ends are reported. The hybridization properties and sequen ce specificity of binding of these deoxynucleic guanidine/DNA chimeras with complementary DNA or RNA are described.