Twenty-five premolars (from Nagoya, Japan <0.1 parts/10(6) F in drinki
ng water) were sampled to determine the fluoride content in imbricatio
n lines of Retzius between the grooves and ridges of perikymata on the
enamel surface. Eight small windows were formed on each surface in gr
oove and ridge regions using an etched microsampling technique. By usi
ng a regression curve, y = ax(-b), fluoride concentrations were compar
ed at depths of 1, 3, 5, 10, 20, 30 and 50 mu m in the perikymata regi
ons. Fluoride concentrations increased gradually from the age of 10 to
12 years on the premolar surfaces, but were significantly higher in 1
2-year-olds or older (erupted teeth) than in 10-year-olds or younger (
unerupted teeth). No obvious difference in fluoride concentrations was
found between males and females. In the outermost enamel (<5 mu m dep
th), fluoride concentrations were significantly higher in the grooves
than the ridges of the perikymata, Comparison of the exponential regre
ssion coefficients (-b) of the fluoride profiles showed a significant
difference between the grooves and ridges. It was concluded that fluor
ide concentrations and profiles were higher in grooves than in ridges
of perikymata, probably because they are naturally porous and are stag
nation areas attracting dental plaque. (C) 1998 Elsevier Science Ltd.
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