IMMOBILIZATION OF RECOMBINANT FIREFLY LUCIFERASE - PHYSICOCHEMICAL PROPERTIES AND APPLICATION

Authors
LUNDOVSKIKH IA DEMENTIEVA EI UGAROVA NN
Citation
Ia. Lundovskikh et al., IMMOBILIZATION OF RECOMBINANT FIREFLY LUCIFERASE - PHYSICOCHEMICAL PROPERTIES AND APPLICATION, Biochemistry, 63(6), 1998, pp. 691-696
Citations number
13
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
63
Issue
6
Year of publication
1998
Pages
691 - 696
Database
ISI
SICI code
0006-2979(1998)63:6<691:IORFL->2.0.ZU;2-X
Abstract
Immobilization of the recombinant Luciola mingrelica and Photinus pyra lis firefly luciferases on BrCN-activated Sepharose was investigated. The catalytic properties and analytical characteristics of the immobil ized recombinant and native luciferases were comparatively studied. Th e catalytic properties of the immobilized recombinant L. mingrelica lu ciferase are close to those of the native luciferase, but the former e nzyme appeared to be significantly more stable. The immobilized recomb inant luciferases can be used for ATP assay within the 0.01-10000 nM r ange.