OECD guidelines and EU directives on the biological testing of chemica
ls contain no methods able to simulate biodegradation in surface water
s. The surface water simulation method presented in this paper is suit
able for closing this gap. The species in the autochthonous biocoenosi
s used in the method form part of the food web in natural surface wate
rs. The microbial degradation activity measured by the half-life is co
mparable with that in surface waters. The degrees of degradation measu
red in this surface water simulation method can be applied to natural
surface waters.