STUDY OF CYTOKININ SEPARATION USING CAPILLARY-ELECTROPHORESIS WITH CYCLODEXTRIN ADDITIVES

The separation of a set of 10 cytokinins by capillary electrophoresis was investigated. The influence of various cyclodextrin derivatives (a lpha-, beta-, gamma-, hydroxypropyl-beta- and dimethyl-beta-cyclodextr ins) in the background electrolyte on the migration characteristics of the compounds investigated was studied. The relationship between the structures of single cytokinins and the type of cyclodextrin used was evaluated. A set of all 10 cytokinins was successfully separated in 10 0 mmol l(-1) phosphate-Tris buffer (pH 2.5) as a background electrolyt e under different experimental conditions e.g., with addition of 25 mm ol l(-1) gamma-cyclodextrin. (C) 1998 Elsevier Science B.V. All rights reserved.