CA2-MODULATED AND MG2+-MODULATED LIPOLYSIS IN NEONATAL RAT-BRAIN SLICES OBSERVED BY ONE-DIMENSIONAL AND 2-DIMENSIONAL NMR()

Superfused cortical brain slices from neonatal rats demonstrated large increases in levels of NMR-detectable lipids after sample preparation and perfusion with standard artificial CSF. These increases were redu ced by an average of 58% by perfusion with buffer with low (no added) Ca2+ or by perfusion in Ca2+-free buffer. Perfusion with buffer with e levated MgSO4 (10 mmol/L) reduced the lipid changes by 47%. A reductio n of 88% was observed in samples perfused in buffer with both low Ca2 and high Mg2+, suggesting a role for Mg2+ in reducing lipolysis disti nct from its known ability to block Ca2+ influx.