A MOLECULAR-MODEL OF MYELIN OLIGODENDROCYTE GLYCOPROTEIN

Myelin oligodendrocyte glycoprotein (MOG) is a protein on the surface of myelin sheaths, It is a putative target of the autoimmune attack in the inflammatory and demyelinating CNS disease multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis. MOG belo ngs to the immunoglobulin superfamily (IgSF), and its extracellular N- terminal domain contains many conserved IgSF consensus residues seen i n immunoglobulin variable region folds. The aim of the present study w as to create a molecular model of the extracellular N-terminal domain of mouse MOG. No crystal structure is yet available of MOG, and thus a molecular model would be useful in providing insight into its structu re and binding characteristics. Molecular graphics techniques and mole cular dynamics with secondary structure-based restraints were used in the construction and refinement of the MOG model, Regions of high pred iction confidence were identified, and possible glycosylation, dimeriz ation, complement binding, and antibody-binding regions in MOG were ma pped and analyzed.