M. Du et al., CLONING AND EXPRESSION ANALYSIS OF A NOVEL SALICYLATE SUPPRESSIBLE GENE, HS-CUL-3, A MEMBER OF CULLIN CDC53 FAMILY/, The Journal of biological chemistry, 273(38), 1998, pp. 24289-24292
By using a mRNA differential display technique to search for salicylat
e suppressible genes, we identified a cDNA in human foreskin fibroblas
ts, which by Gen-Bank(TM) DNA data base search shows sequence homology
to the recently reported cullin/Cdc53 (CUL) family genes, especially
CUL-3, We have cloned the full-length human CUL-3 (Hs-CUL-3) cDNA, It
encodes a 768-amino acid polypeptide and has a predicted molecular wei
ght of 88,939, The amino acid sequence of Hs-CUL-3 shows 46% homology
to that of its Caenorhabditis elegans ortholog, Ce-CUL-3, and 27 and 2
3% to that of Hs-CUL-1 and Hs-CUL-2, respectively. Northern blot analy
sis showed that phorbol 12-myristate 13-acetate increased the expressi
on of Hs-CUL-3 mRNA in a concentration- and time-dependent manner, and
this increase was inhibited by sodium salicylate, Hs-CUL-3 widely exp
ressed in human tissues and its expression in cultured COLO205 colon c
ancer cells was increased when compared with that in normal colon cell
s, It is Likely that Hs-CUL-3 is involved in cell proliferation contro
l.