APPLICATION IN THE DAMAGE OF DNA BY ELECTROCHEMICAL DNA SENSOR

Mutation and damage in DNA by interaction, UV-irralation, nitrite acti on and ultrasonication were studied by means of voltammetry using a gr aphite electrode covalently immobilied with ssDNA-1, Daunomycin was us ed as a hybidization indicator. When the complementary ssDNA-2 in solu tion was damaged by phsical or chemical agents, the hybridization of s sDNA-1 and ssDNA-2 was changed, All the results in voltammogram indica ted that intercalator can't destroy the double-helical structure of DN A, while UV-irralation and nitrite action can make the permanent damag e, Ultrasonication can reduce polymerization in DNA and the hybridizat ion time was shortened. This may provide a new method for clinical med icine study.