Bs. Seidel et W. Faubel, FIBER OPTIC MODIFIED THERMAL LENS DETECTOR SYSTEM FOR THE DETERMINATION OF AMINO-ACIDS, Journal of chromatography, 817(1-2), 1998, pp. 223-226
Citations number
15
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A novel fiber optic modified thermal lens detector in combination with
capillary electrophoresis (CE) is described. The probe and pump laser
s used in thermal lensing were adapted via optical fibers to a commerc
ial injection and high voltage CE system. The detector performance is
demonstrated by monitoring mixtures of derivatised amino acids. Amino
acids are labeled with 4-dimethylazobenzene-4-sulfonyl-chloride as abs
orbance reagent. Therefore all amino acids possess a large molar absor
ptivity in the visible region which because of its stability can be qu
alitatively and quantitatively detected with only one excitation line.
The miniaturization of the thermal lens detector and the separation o
f the excitation and probe laser from the sensor head with optical wav
eguides permits combination with typical laboratory CE systems, which
generally allow treatment of microliter volumes of amino acids. (C) 19
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