Ma. Roberts et al., SEPARATION OF HIGH-MOLECULAR-MASS CARRAGEENAN POLYSACCHARIDES BY CAPILLARY-ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION, Journal of chromatography, 817(1-2), 1998, pp. 353-366
Citations number
33
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A method is described for the separation and quantitation of unhydroly
zed carrageenan polysaccharides (molecular mass approx. 3.10(5) rel. m
ol mass) by capillary electrophoresis. Various carrageenan samples wer
e first derivatized with the fluorescent reagent, trisodium 8-aminopyr
ene-1,3,6-trisulfonate (APTS), in both laboratory and commercial blend
samples. Microcentrifuge filters (3.10(4) rel. mel. mass) were then e
mployed to separate derivatized samples into low (eluate) and high (re
tentate) molecular mass fractions which could each be assayed by CE wi
th laser-induced fluorescence detection. Eluate fractions contained sm
all amounts of kappa and iota species which could be efficiently separ
ated by capillary zone electrophoresis (CZE) using a buffer with an an
ti-convective additive. Separation of species contained in the retenta
te fraction (kappa, iota, lambda) could be achieved in under 5 min by
CZE using a citrate buffer, pH 3.0. The influence of the pre-filtratio
n step, field strength and temperature on peak efficiency and resoluti
on are studied. Quantitative aspects are evaluated and the method then
applied to real commercial samples of food additive mixtures. (C) 199
8 Published by Elsevier Science B.V.