A COMPARISON BETWEEN ELISA AND DISSECTION FOR DETECTION OF PLASMODIUMINFECTIONS IN ANOPHELES MOSQUITOS - CONSEQUENCES OF THE ESTIMATION OFMALARIA TRANSMISSION IN NDIOP, SENEGAL IN 1995

An entomological study was carried out in Ndiop village, Senegal, an a rea of sudan-type savana, from January io December 1995, io compare th e malaria inoculation rate measured by the dissection oi salivary glan ds of anophelines and the enzyme-linked immunosorbent assay (ELISA). M osquitoes were sampled by night-bite collections, Species from the Ano pheles gambiae complex were identified using the polymerase chain reac tion. 1292 females Culicidae were collected. 597 anophelines mosquitoe s known to be malaria vectors were captured during 148 man-nights of c apture. Of the 572 anophelines investigated, 14 were positive using di ssection while 21 were found to be positive with ELISA. The sporozoite rates were 2.5 %, 2.2 % and 3.8 % for An. arabiensis, An. gambiae and An. funestus, respectively. The circumsporozoite rates were 3.5 %, 2. 2% and 7.7 % far these three species, respectively. ELISA detected 1.5 times more positive mosquitoes than dissection. These results ore dis cussed with reference to the duration of the sporogonic cycle, the del ay before CSP antigens are apparent, and the dolly survival rate of An opheles mosquitoes.