Two cases of cutaneous Pythiosis insidiosi were diagnosed in cattle fr
om the Pantanal region, Brazil. The lesions were observed in the limbs
of two 8-month-old beef calves. Close examination showed local swelli
ng and focal ulceration of the skin. Microscopically, there nias multi
focal granulomatous dermatitis with intralesional Pythium insidiosum h
yphae. The diagnosis was based on the morphological aspects, immunohis
tochemical findings and culture of the etiologic agent.