MAPPING AN ENDOMETRIAL CANCER TUMOR-SUPPRESSOR GENE AT 10Q25 AND DEVELOPMENT OF A BACTERIAL CLONE CONTIG FOR THE CONSENSUS DELETION INTERVAL

Frequent loss of chromosome 10q sequences in endometrial cancers sugge sts the involvement of a tumor suppressor gene. Previous loss-of-heter ozygosity (LOH) studies have pointed to the 10q25-q26 region as the li kely site of a tumor suppressor involved in endometrial tumorigenesis (S. L. Peiffer et al,, 1995, Cancer Res. 55: 1922-1926; S. Nagase et n l,. 1996, Br. J. Cancer 74: 1979-1983; 1997, Cancer Res. 57: 1630-1633 ). In an attempt to define further the localization of a tumor suppres sor gene at 10q25, we screened a panel of 123 endometrioid adenocarcin omas for loss of heterozygosity of 10q25.3 sequences. Forty-three (35% ) revealed LOH at one or more loci, The observed patterns of allelic l oss define a minimum consensus region of deletion between D10S221 and D10S610, A sequence-ready bacterial clone contig and a long-range rest riction map for a 1-Mb interval spanning the deletion region were deve loped as the first step in experiments directed toward the discovery t he 10q25 tumor suppressor. (C) 1998 Academic Press.