Z. Bercovich et al., ESTIMATION OF THE BIOLOGICAL-ACTIVITY (POTENCY) OF BATCHES OF BRUCELLIN PREPARED FROM A MUCOID STRAIN OF BRUCELLA-ABORTUS, Veterinary microbiology, 62(4), 1998, pp. 313-320
A study was conducted to determine the repeatability of a procedure us
ed to prepare brucellin from a mucoid strain of Brucella abortus, and
to determine the biological activity of those brucellins, The brucelli
ns were standardized to contain 1 mg protein/ml, and their potency was
estimated according to the European pharmacopoeia norm for tuberculin
. Estimation of the potency was done in cattle that have been sensitiz
ed with living or killed brucellae, A brucellin that effectively detec
ted acute and chronic brucellosis in cattle with experimentally induce
d brucellosis was used as reference brucellin, Results show that five
of the nine batches of brucellin equalled the potency of the reference
brucellin. Three brucellins had 79-88% potency of the reference bruce
llin and one had only 59%. Since the potency of various brucellins may
vary it is suggested to estimate the potency of the brucellins accord
ing to the European pharmacopoeia norm for tuberculin. (C) 1998 Elsevi
er Science B.V. All rights reserved.