VANADIUM INDUCES AP-1-DEPENDENT AND NF-KAPPA-B-DEPENDENT TRANSCRIPTION ACTIVITY

Vanadate has been reported to be involved in the causation of cancer. In this study, we found that both AP-1 and NF kappa-B activities were increased after treatment with sodium vanadate in JB6 cells. Maximum i nduction of AP-1 and NF kappa B appeared at 48 to 72 h. Phosphorylatio ns of Erks and p38 kinases were markedly increased at 100 mu M of vana date. while phosphorylation of JNKs was not affected, Vanadate also en hances the phosphorylation of I kappa B alpha. These results suggest t hat the activation of AP-1 and NF kappa B by vanadate may be mediated through enhancement of phosphorylation of Erk/p38 kinases and I kappa B alpha. respectively.