REBAMIPIDE PREVENTED DELAY OF WOUND REPAIR INDUCED BY HYDROGEN-PEROXIDE AND SUPPRESSED APOPTOSIS OF GASTRIC EPITHELIAL-CELLS IN-VITRO

The effects of rebamipide on the restoration process of gastric epithe lial wounds were assessed using an in vitro wound-healing model and hy drogen peroxide treatment. Rebamipide (10 or 100 mu M) was added to a complete monolayer cell sheet after artificial wounding. The restorati on process was analyzed sequentially by a time-lapse video system, and cell migration, proliferation, and apoptosis were assessed. Hydrogen peroxide (1 or 3 mM) inhibited restoration after wounding by suppressi ng cell migration and proliferation. It also induced epithelial cell a poptosis around the wound. The addition of rebamipide abolished the H2 O2-induced retardation and prevented apoptosis, Rebamipide might act a s a radical scavenger and have favorable effects on peptic ulcer disea ses.