SODIUM DODECYL SULFATE-CAPILLARY ELECTROPHORESIS OF PROTEINS IN A SIEVING MATRIX UTILIZING 2-SPECTRAL CHANNEL LASER-INDUCED FLUORESCENCE DETECTION

Authors
CRAIG DB POLAKOWSKI PM ARRIAGA E WONG JCY AHMADZADEH H STATHAKIS C DOVICHI NJ
Citation
Db. Craig et al., SODIUM DODECYL SULFATE-CAPILLARY ELECTROPHORESIS OF PROTEINS IN A SIEVING MATRIX UTILIZING 2-SPECTRAL CHANNEL LASER-INDUCED FLUORESCENCE DETECTION, Electrophoresis, 19(12), 1998, pp. 2175-2178
Citations number
11
Categorie Soggetti
Biochemical Research Methods","Chemistry Analytical
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
19
Issue
12
Year of publication
1998
Pages
2175 - 2178
Database
ISI
SICI code
0173-0835(1998)19:12<2175:SDSEOP>2.0.ZU;2-6
Abstract
We report a method for protein labeling, separation by capillary elect rophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample pr oteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, i rrespective of variations in run-to-run migration times.