NONAQUEOUS CAPILLARY-ZONE-ELECTROPHORESIS FOR SEPARATION OF FREE FATTY-ACIDS WITH INDIRECT FLUORESCENCE DETECTION

The feasibility of combining nonaqueous capillary zone electrophoresis with indirect fluorescence detection was studied for the efficient se paration and sensitive detection of ionizable hydrophobic substances w hich do not possess practically suitable detection properties. Medium- and long-chain free fatty acids, C-6-C-24, were selected as test comp ounds. The results showed that such a wide range of medium- and long-c hain free fatty acids could be separated between any two consecutive h omologs in one run and be detected at a level of about 0.01-0.02 mM in highly basic methanol/acetonitrile media containing fluorescein as co lon of background electrolyte for indirect fluorescence detection.